Progress in the radiolabeling of Mycobacterium leprae.
نویسنده
چکیده
منابع مشابه
A RAPID AND SIMPLE METHOD OF DNA EXTRACTION FROM CLINICAL SPECIMENS CONTAINING MYCOBACTERIUM LEPRAE FOR PCR ASSAYS
We describe a rapid, simple, and reliable procedure for routine isolation of Mycobacterium leprae DNA from slit-skin swab specimens. This one-step DNA extraction method is based on the utilization of ChelexR 100, a chelating ion exchange resin. Slit-skin swab specimens from seven untreated leprosy patients at Baba Baghi Leprosy Hospital in Iran were processed by this procedure. The polymer...
متن کاملRapid, radiolabeled-microculture method that uses macrophages for in vitro evaluation of Mycobacterium leprae viability and drug susceptibility.
This paper describes a microculture rapid assay using radiolabeling and mouse macrophages to determine the viability and the drug susceptibility or resistance of Mycobacterium leprae. Comparison of M. leprae resident macrophage cultures maintained in 96-well flat-bottomed plates showed results for viability and susceptibility or resistance to dapsone that were similar to results for concurrent ...
متن کاملUse of 18 and 36 kDa antigens of Mycobacterium Leprae for detection of this microorganism in Iranian Leprosy patients by PCR
.
متن کاملDetection of Non-Tuberculosis Mycobacteria Infection due to Mycobacterium leprae and Mycobacterium kansasii in Patients Suspected of Tuberculosis in Isfahan, Iran
Several reports have indicated that infection with Non-Tuberculosis Mycobacteria (NTM) is increasing worldwide.Therefore,monitoring species causing micobacterial infection in any region is of great importance. This study was going to detect, differentiate, and identify pathogenic mycobacteria in primary clinical samples. Eighty samples collected from tuberculosis suspected patients in Isfahan/I...
متن کاملTHE DETECTION OF Mycobacterium tuberculosis BY PCR IN SPUTUM SAMPLES FROM LONG-TERMTREATED LEPROSY PATIENTS: ASSOCIATIONS WITH SKIN TEST RESULTS AND IMMUNOTHERAPY WITH Mycobacterium vaccae
As part of a series of investigations at Baba Baghi Leprosarium in lran, 44 long-treated leprosy patients were selected for our study. Samples of early morning sputum were obtained from each patient, examined by microscopy for acid-fast bacilli (AFB), and cultured for tubercle bacilli. These tests were negative, but the polymerase chain reaction (PCR) for an insertion sequence believed to b...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association
دوره 44 1-2 شماره
صفحات -
تاریخ انتشار 1976